Cryptotanshinone possesses therapeutic effects on ischaemic stroke through regulating STAT5 in a rat model
Context: Cryptotanshinone (CT), a lipophilic compound obtained from roots of Salvia miltiorrhiza Bunge (Lamiaceae) (Danshen), has multiple qualities in illnesses, for example lung fibrosis, cancer of the lung, and osteo arthritis. Our previous findings claim that CT plays a safety role in cerebral stroke. However, the molecular mechanisms underlying CT protection in ischaemic stroke remain unclear.
Objective: This research examines the result of CT on ischaemic stroke.
Materials and techniques: We used the center cerebral artery occlusion (MCAO) rat (Sprague-Dawley rats, 200 ± 20 g, n = 5) model having a sham operation group was treated as negative control. MCAO rats were given 15 mg/kg CT using intragastric administration. Furthermore, TGF-ß (5 ng/mL) was utilized to deal with MCAO rats like a positive control group.
Results: The 50% inhibitory concentration (IC50) of CT on CD4 cell STAT5-IN-1 damage was 485.1 µg/mL, and median effective concentration (EC50) was 485.1 µg/mL. CT attenuates the infarct region within the MCAO model. The proportion of CD4 CD25 FOXP3 Treg cells within the peripheral bloodstream from the MCAO group was elevated with CT treatment. The protein degree of FOXP3 and also the phosphorylation of STAT5 were retrieved within the CD4 CD25 Treg cells of model group after given CT. Importantly, the results of CT treatment were blocked by treatment using the inhibitor STAT5-IN-one in CD4 T cells from the MCAO model.
Discussion and conclusion: Our findings not just boost the knowledge of the mechanisms underlying CT treatment, but additionally indicate its potential value like a promising agent in treating ischaemic stroke. Further study is going to be valuable to look at the results of CT on patients with ischaemic stroke.